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Literature summary for 2.3.3.10 extracted from

  • Skaff, D.A.; Ramyar, K.X.; McWhorter, W.J.; Barta, M.L.; Geisbrecht, B.V.; Miziorko, H.M.
    Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin (2012), Biochemistry, 51, 4713-4722.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with hymeglusin, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.3-6.5), 0.2 M NaCl, and 23% (w/v) polyethylene glycol 3350 Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
hymeglusin i.e. (2E,4E,7R)-11-[(2R,3R)-3-(hydroxymethyl)-4-oxooxetan-2-yl]-3,5,7-trimethylundeca-2,4-dienoic acid, time-dependent loss of activity is observed with more than 95% inhibition achieved at extended incubation times (10 min at 75-600 nM) Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q9FD71
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA Sepharose column chromatography and Resource Q anion-exchange column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Synonyms

Synonyms Comment Organism
HMG-CoA synthase
-
 Enterococcus faecalis
mvaS
-
 Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
 hymeglusin in 100 mM Tris-HCl (pH 8.0), at 18°C Enterococcus faecalis