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Literature summary for 2.3.3.10 extracted from

  • Sirinupong, N.; Suwanmanee, P.; Doolittle, R.F.; Suvachitanont, W.
    Molecular cloning of a new cDNA and expression of 3-hydroxy-3-methylglutaryl-CoA synthase gene from Hevea brasiliensis (2005), Planta, 221, 502-512.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Hevea brasiliensis
HMGS1, expression in Escherichia coli Hevea brasiliensis

Protein Variants

Protein Variants Comment Organism
C117A Km-value for acetyl-CoA is 1.7fold higher than the wild-type value, specific activity is 30% of wild-type activity Hevea brasiliensis
N326A Km-value for acetyl-CoA is 1.6fold higher than the wild-type value, specific activity is 5% of wild-type activity Hevea brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 acetyl-CoA wild-type enzyme Hevea brasiliensis
0.84
-
 acetyl-CoA mutant enzyme N326A Hevea brasiliensis
0.9
-
 acetyl-CoA mutant enzyme C117A Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
laticifer HMGS2 is well expressed Hevea brasiliensis
-
leaf HMGS2 is poorly expressed Hevea brasiliensis
-
petiole HMGS2 is well expressed Hevea brasiliensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Hevea brasiliensis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl-CoA synthase
-
 Hevea brasiliensis
HMGS1
-
 Hevea brasiliensis
HMGS2
-
 Hevea brasiliensis