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Literature summary for 2.3.3.10 extracted from
- 3-Hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1988), Methods Enzymol., 110, 19-26.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA | - |
Gallus gallus |  |
| acetoacetyl-CoA | - |
Gallus gallus | |
| DL-3-hydroxy-3-methylglutaryl-CoA | competitive inhibition | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00035 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
| 0.019 | - |
S-(3-oxobutyl)-CoA | - |
Gallus gallus | |
| 0.1 | - |
acetyl-CoA | - |
Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Gallus gallus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
2 * 53000, SDS-PAGE | Gallus gallus |
| 105000 | - |
sedimentation equlibrium | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
chicken | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.85 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, in presence of 30% glycerol stable for over 1 year | Gallus gallus |
| 4°C, in presence of 30% glycerol stable for several days | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxobutyryl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | 3-hydroxy-3-methyl-5-oxoheptanedioyl-CoA + CoA | - |
? | |
| acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
| acetyl-CoA + N-acetyl-S-acetoacetylcysteamine + H2O | - |
Gallus gallus | ? + CoA | - |
? | |
| H2O + acetyl-CoA + S-(3-oxobutyl)-CoA | - |
Gallus gallus | CoA + S-(4-carboxy-3-hydroxy-isoamyl)-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, SDS-PAGE | Gallus gallus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
| 0.012 | - |
DL-3-hydroxy-3-methylglutaryl-CoA | - |
Gallus gallus | |
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