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Literature summary for 2.3.3.10 extracted from
- The kinetic mechanism of 3-hydroxy-3-methylglutaryl-coenzyme a synthase from baker's yeast (1972), Biochem. J., 126, 35-47.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3-dimethylglutaryl-CoA | - |
Saccharomyces cerevisiae |  |
| 3-oxohexanoyl-CoA | - |
Saccharomyces cerevisiae | |
| acetoacetyl-CoA | substrate inhibition | Saccharomyces cerevisiae | |
| butyryl-CoA | - |
Saccharomyces cerevisiae | |
| CoA | - |
Saccharomyces cerevisiae | |
| decanoyl-CoA | - |
Saccharomyces cerevisiae | |
| desulfo-CoA | - |
Saccharomyces cerevisiae | |
| DL-3-hydroxy-3-methylglutaryl-CoA | uncompetitive inhibition | Saccharomyces cerevisiae | |
| DL-3-methylglutaryl-CoA | - |
Saccharomyces cerevisiae | |
| glutaryl-CoA | - |
Saccharomyces cerevisiae | |
| heptanoyl-CoA | - |
Saccharomyces cerevisiae | |
| hexanoyl-CoA | - |
Saccharomyces cerevisiae | |
| nonanoyl-CoA | - |
Saccharomyces cerevisiae | |
| octanoyl-CoA | - |
Saccharomyces cerevisiae | |
| propionyl-CoA | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
acetoacetyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.0032 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
| 0.014 | - |
acetyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.018 | - |
acetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | synthesis of isoprenes and steroids | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
| acetyl-CoA + acetoacetyl-CoA + H2O | synthesis of isoprenes and steroids | Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
| additional information | acetyl-CoA homologues do not act as substrates | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | absolutely specific for acetyl-CoA and acetoacetyl-CoA in the reaction, neither propionyl- nor butyryl-CoA can replace acetyl-CoA, no reaction observed when 3-oxohexanoyl-CoA is substituted for acetoacetyl-CoA | Saccharomyces cerevisiae | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
decanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.004 | - |
nonanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.005 | - |
propionyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.0071 | - |
3-oxohexanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.008 | - |
acetoacetyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.0081 | - |
octanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.0123 | - |
heptanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.013 | - |
DL-3-hydroxy-3-methylglutaryl-CoA | pH 8.0, competitive inhibition, substrate acetyl-CoA | Saccharomyces cerevisiae | |
| 0.014 | - |
3-oxohexanoyl-CoA | pH 8.0, mixed inhibition, substrate acetoacetyl-CoA | Saccharomyces cerevisiae | |
| 0.02 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
| 0.022 | - |
hexanoyl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.03 | - |
desulfo-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.033 | - |
butyryl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.038 | - |
CoA | pH 8.0, mixed inhibition, substrate acetyl-CoA | Saccharomyces cerevisiae | |
| 0.06 | - |
CoA | pH 8.0, mixed inhibition, substrate acetoacetyl-CoA | Saccharomyces cerevisiae | |
| 0.07 | - |
desulfo-CoA | pH 8.0, mixed inhibition, substrate acetoacetyl-CoA | Saccharomyces cerevisiae | |
| 0.1 | - |
DL-3-methylglutaryl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.11 | - |
glutaryl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
| 0.12 | - |
3,3-dimethylglutaryl-CoA | pH 8.0 | Saccharomyces cerevisiae | |
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