Literature summary for 2.3.2.B14 extracted from

Erdemli, S.B.; Gupta, R.; Bishai, W.R.; Lamichhane, G.; Amzel, L.M.; Bianchet, M.A.
Targeting the cell wall of Mycobacterium tuberculosis: structure and mechanism of L,D-transpeptidase 2 (2012), Structure, 20, 2103-2115.

Search for more literature for 2.3.2.B14

Cloned(Commentary)

Cloned (Comment)	Organism
the extramembrane portion of the L,D-transpeptidase is expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 1 M succinic acid, and 1% (w/v) PEG MME 2000	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
C354A	inactive	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
ertapenem	-	Mycobacterium tuberculosis
Imipenem	irreversible inhibition, imipenem binds only the catalytically active enzyme with apparent submicromolar affinity	Mycobacterium tuberculosis
meropenem	meropenem binds only the catalytically active enzyme with apparent submicromolar affinity	Mycobacterium tuberculosis
additional information	not inhibited by clavulanic acid	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Mycobacterium tuberculosis	5618	-

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O53223	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography and Sepharose 12 column chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme also shows low beta-lactamase activity with nitrocefin (i.e. 3-(2,4-dinitrostyryl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid) with Km of 0.016 mM and kcat of 0.01 s-1 (in 20 mM HEPES (pH 7.5) and 300 mM NaCl, temperature not specified in the publication)	Mycobacterium tuberculosis	?	-	?

Subunits

Subunits	Comment	Organism
monomer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
L,D-transpeptidase 2	-	Mycobacterium tuberculosis
LdtMt2	-	Mycobacterium tuberculosis
MT0125	-	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	pH optimum for beta-lactamase activity with nitrocefin	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)