Crystallization (Comment) | Organism |
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crystal structure of Ufd2 in complex with the ubiquitin-like (UBL) and ubiquitin-associated (UBA) proteins Rad23 and Dsk2 domains, PDB-ID: 3M62 and 3M63, respectively. The structure of Ufd2 is solved in complex with Rad23-UBL carrying either an N-terminal or a C-terminal His tag, refined at 2.4 A resolution. Ufd2 is composed of an N-terminal variable domain, a core domain, and a C-termional U-box with a fold similar to that of RING. The structure of Ufd2 with Dsk2-UBL is solved by molecular replacement. THe structure exhibits increased flexibility, in particular with a C-terminally tagged UBL domain. The N-terminal, tagged protein structure is refined at 2.4 A resolution. The N-terminal UBL-binding region of yeast Ufd2 is conserved on lower eukaryotes | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P54860 | crystallization: structure of Ufd2 in complex with the ubiquitin-like (UBL) and ubiquitin-associated (UBA) proteins Rad23 and Dsk2 domains, PDB-ID: 3M62 and 3M63, respectively; S288c | - |
Saccharomyces cerevisiae ATCC 204508 | P54860 | crystallization: structure of Ufd2 in complex with the ubiquitin-like (UBL) and ubiquitin-associated (UBA) proteins Rad23 and Dsk2 domains, PDB-ID: 3M62 and 3M63, respectively; S288c | - |
Purification (Comment) | Organism |
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- |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
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E4 ubiquitin-protein ligase UFD2 | - |
Saccharomyces cerevisiae |
ubiquitin conjugation factor E4 | - |
Saccharomyces cerevisiae |
ubiquitin fusion degradation protein 2 | - |
Saccharomyces cerevisiae |
Ufd2 | - |
Saccharomyces cerevisiae |