Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.8 extracted from

  • Kato, M.
    Heparin as an inhibitor of L-arginyl-tRNA: protein arginyltransferase (1983), J. Biochem., 94, 2015-2022.
    View publication on PubMed
Search for more literature for 2.3.2.8

Inhibitors

Inhibitors Comment Organism Structure
heparin 50% inhibition at 0.05 mg/ml, kinetics, competitive to L-arginyl-tRNA Rattus norvegicus
heparin 50% inhibition at 0.05 mg/ml, kinetics, competitive to L-arginyl-tRNA Saccharomyces cerevisiae
heparin 50% inhibition at 0.05 mg/ml, kinetics, competitive to L-arginyl-tRNA Sus scrofa
additional information no inhibition by chondroitinsulfate A, B or C, hyaluronic acid, D-glucosamine N-sulfate, D-glucose 6-sulfate, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-xylose, D-glucuronic acid Rattus norvegicus
additional information no inhibition by chondroitinsulfate A, B or C, hyaluronic acid, D-glucosamine N-sulfate, D-glucose 6-sulfate, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-xylose, D-glucuronic acid Saccharomyces cerevisiae
additional information no inhibition by chondroitinsulfate A, B or C, hyaluronic acid, D-glucosamine N-sulfate, D-glucose 6-sulfate, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-xylose, D-glucuronic acid Sus scrofa
putrescine above 30 mM Sus scrofa
spermidine competitive to L-arginyl-tRNA Rattus norvegicus
spermidine competitive to L-arginyl-tRNA Saccharomyces cerevisiae
spermidine competitive to L-arginyl-tRNA Sus scrofa
spermine competitive to L-arginyl-tRNA Rattus norvegicus
spermine competitive to L-arginyl-tRNA Saccharomyces cerevisiae
spermine competitive to L-arginyl-tRNA Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 arginyl-tRNA
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble Rattus norvegicus 5737
-
cytoplasm soluble Sus scrofa 5737
-
cytoplasm soluble Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginyl-tRNAArg + acceptor protein Rattus norvegicus
-
 tRNAArg + L-arginyl-[acceptor protein]
-
 ?
L-arginyl-tRNAArg + acceptor protein Sus scrofa
-
 tRNAArg + L-arginyl-[acceptor protein]
-
 ?
L-arginyl-tRNAArg + acceptor protein Saccharomyces cerevisiae
-
 tRNAArg + L-arginyl-[acceptor protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Sus scrofa
-
 hog
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on heparin-Sepharose, homogeneity Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
kidney
-
 Sus scrofa
-
spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 after addition of inhibitors during purification Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginyl-tRNAArg + acceptor protein
-
 Rattus norvegicus tRNAArg + L-arginyl-[acceptor protein]
-
 ?
L-arginyl-tRNAArg + acceptor protein
-
 Sus scrofa tRNAArg + L-arginyl-[acceptor protein]
-
 ?
L-arginyl-tRNAArg + acceptor protein
-
 Saccharomyces cerevisiae tRNAArg + L-arginyl-[acceptor protein]
-
 ?
L-arginyl-tRNAArg + alpha lactalbumin
-
 Sus scrofa tRNAArg + L-arginyl-[lactalbumin]
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
 spermine
-
 Sus scrofa
0.0015
-
 heparin
-
 Sus scrofa