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Literature summary for 2.3.2.8 extracted from
- Enzymatic modification of proteins. II. Purification and properties of the arginyl transfer ribonucleic acid-protein transferase from rabbit liver cytoplasm (1970), J. Biol. Chem., 245, 731-737.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | requirement, 0.1 M, stabilizes the enzyme in a reduced state | Oryctolagus cuniculus |  |
| dithiothreitol | requirement, 0.01 M stabilizes the enzyme in a reduced state | Oryctolagus cuniculus | |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol or dithiothreitol stabilize during storage | Oryctolagus cuniculus |
| glycerol, 20%, stabilizes concentrated enzyme solutions during storage | Oryctolagus cuniculus |
| polyethylene glycol, 30%, stabilizes concentrated solutions during storage | Oryctolagus cuniculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble | Oryctolagus cuniculus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | requirement, optimal concentration is 0.2 M | Oryctolagus cuniculus | |
| K+ | requirement for monovalent cations | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginyl-tRNAArg + acceptor protein | Oryctolagus cuniculus | - |
tRNAArg + L-arginyl-[acceptor protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7000fold | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.114 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, concentrated enzyme solution, 20% glycerol, t1/2: about 1 week | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginyl-tRNAArg + acceptor protein | - |
Oryctolagus cuniculus | tRNAArg + L-arginyl-[acceptor protein] | - |
? | |
| L-arginyl-tRNAArg + albumin | from bovine serum, accepts 1 mol arginine/mol | Oryctolagus cuniculus | tRNAArg + L-arginyl-albumin | - |
? | |
| L-arginyl-tRNAArg + fructose diphosphatase | from rabbit liver | Oryctolagus cuniculus | tRNAArg + L-Arg-[fructose diphosphatase] | - |
? | |
| L-arginyl-tRNAArg + insulin | less effective | Oryctolagus cuniculus | tRNAArg + L-arginyl-[insulin] | - |
? | |
| L-arginyl-tRNAArg + thyroglobulin | accepts 2 mol arginine/mol | Oryctolagus cuniculus | tRNAArg + L-arginyl-[thyroglobulin] | - |
? | |
| L-arginyl-tRNAArg + transaldolase | I and III from Candida | Oryctolagus cuniculus | tRNAArg + L-arginyl-[transaldolase] | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | 9.4 | - |
Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.8 | about half-maximal activity at pH 8.0, about 80% of maximal activity at pH 9.8 | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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