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Literature summary for 2.3.2.6 extracted from

  • Leibowitz, M.J.; Soffer, R.L.
    Enzymatic modification of proteins. 3. Purification and properties of a leucyl, phenylalanyl transfer ribonucleic acid protein transferase from Escherichia coli (1970), J. Biol. Chem., 245, 2066-2073.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 20 mM, 35% inhibition Escherichia coli
Mg2+ 50 mM, 80% inhibition Escherichia coli
MnCl2 91% inhibition Escherichia coli
puromycin aminonucleoside derivative, which lacks the aminoacyl analogus structure, fails to inhibit the reaction Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation required, maximal activity is 0.15 M KCl for the transfer of Phe and 0.2 M for the transfer of Leu Escherichia coli
additional information no Mg2+-requirement Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for at least 2 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucyl-tRNALeu + protein acceptor protein: bovine serum albumin Escherichia coli tRNALeu + L-leucyl-protein
-
?
L-leucyl-tRNALeu + protein incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli tRNALeu + L-leucyl-protein
-
?
L-leucyl-tRNALeu + protein acceptor protein: bovine serum albumin Escherichia coli B / ATCC 11303 tRNALeu + L-leucyl-protein
-
?
L-leucyl-tRNALeu + protein incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli B / ATCC 11303 tRNALeu + L-leucyl-protein
-
?
L-phenylalanyl-tRNA + acceptor protein incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli tRNA + L-phenylalanyl-protein
-
?
L-phenylalanyl-tRNA + acceptor protein incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli B / ATCC 11303 tRNA + L-phenylalanyl-protein
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
t1/2: 1.5 min, complete inactivation after 8.5 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.2 transfer of Leu Escherichia coli
8.2 8.6 transfer of Phe Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 80% of maximal activity at pH 7.0 and 9.0 Escherichia coli