Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 20 mM, 35% inhibition | Escherichia coli | |
Mg2+ | 50 mM, 80% inhibition | Escherichia coli | |
MnCl2 | 91% inhibition | Escherichia coli | |
puromycin | aminonucleoside derivative, which lacks the aminoacyl analogus structure, fails to inhibit the reaction | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required, maximal activity is 0.15 M KCl for the transfer of Phe and 0.2 M for the transfer of Leu | Escherichia coli | |
additional information | no Mg2+-requirement | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucyl-tRNALeu + protein | acceptor protein: bovine serum albumin | Escherichia coli | tRNALeu + L-leucyl-protein | - |
? | |
L-leucyl-tRNALeu + protein | incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin | Escherichia coli | tRNALeu + L-leucyl-protein | - |
? | |
L-leucyl-tRNALeu + protein | acceptor protein: bovine serum albumin | Escherichia coli B / ATCC 11303 | tRNALeu + L-leucyl-protein | - |
? | |
L-leucyl-tRNALeu + protein | incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin | Escherichia coli B / ATCC 11303 | tRNALeu + L-leucyl-protein | - |
? | |
L-phenylalanyl-tRNA + acceptor protein | incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin | Escherichia coli | tRNA + L-phenylalanyl-protein | - |
? | |
L-phenylalanyl-tRNA + acceptor protein | incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin | Escherichia coli B / ATCC 11303 | tRNA + L-phenylalanyl-protein | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
t1/2: 1.5 min, complete inactivation after 8.5 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.2 | transfer of Leu | Escherichia coli |
8.2 | 8.6 | transfer of Phe | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 80% of maximal activity at pH 7.0 and 9.0 | Escherichia coli |