Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells (isoform isoDromeQC) | Drosophila melanogaster |
expressed in Pichia pstoris strain X33 (isoform DromeQC) | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 10% (w/v) PEG 35000 and 100 mM Tris (pH 8.5) for isoform DromeQC | Drosophila melanogaster |
hanging drop vapor diffusion method, using 200 mM MnCl2, 30% (w/v) PEG 4000, and 100 mM Tris (pH 8.5) for isoform isoDromeQC | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
C113A/C136A | mutant of isoform DromeQC lacking a disulfide bond, without influence on the stability of the enzyme | Drosophila melanogaster |
C136A/C158A | mutant of isoform isoDromeQC lacking a disulfide bond, without influence on the stability of the enzyme | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Methylimidazole | - |
Drosophila melanogaster | |
benzimidazole | - |
Drosophila melanogaster | |
benzylimidazole | - |
Drosophila melanogaster | |
imidazole | - |
Drosophila melanogaster | |
Nomega-acetylhistamine | - |
Drosophila melanogaster | |
PBD150 | potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea; potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | isoform DromeQC | Drosophila melanogaster | - |
- |
mitochondrion | isoform isoDromeQC | Drosophila melanogaster | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
39SrpL21 protein | Drosophila melanogaster | - |
? | - |
? | |
Acp33A protein | Drosophila melanogaster | - |
? | - |
? | |
ALDH2 protein | Drosophila melanogaster | - |
? | - |
? | |
DIM-10 protein | Drosophila melanogaster | - |
? | - |
? | |
MCCB protein | Drosophila melanogaster | - |
? | - |
? | |
NPLP4 protein | Drosophila melanogaster | - |
? | - |
? | |
rho-7 protein | Drosophila melanogaster | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q86PD7 | isoform isoDromeQC | - |
Drosophila melanogaster | Q9VRQ9 | isoform DromeQC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
39SrpL21 protein | - |
Drosophila melanogaster | ? | - |
? | |
Acp33A protein | - |
Drosophila melanogaster | ? | - |
? | |
ALDH2 protein | - |
Drosophila melanogaster | ? | - |
? | |
DIM-10 protein | - |
Drosophila melanogaster | ? | - |
? | |
L-Gln-2-naphthylamide | - |
Drosophila melanogaster | 5-oxoprolyl-2-naphthylamide + NH3 | - |
? | |
L-Gln-7-amido-4-methylcoumarin | - |
Drosophila melanogaster | 5-oxoprolyl-7-amido-4-methylcoumarin + NH3 | - |
? | |
L-Gln-Gly | - |
Drosophila melanogaster | 5-oxoprolylglycine + NH3 | - |
? | |
L-Gln-Gly-L-Pro | - |
Drosophila melanogaster | 5-oxoprolyl-Gly-L-Pro + NH3 | - |
? | |
L-Gln-L-Lys-L-Arg-L-Leu | - |
Drosophila melanogaster | 5-oxoprolyl-L-Lys-L-Arg-L-Leu + NH3 | - |
? | |
L-Gln-L-Phe-L-Ala | - |
Drosophila melanogaster | 5-oxoprolyl-L-Phe-L-Ala + NH3 | - |
? | |
MCCB protein | - |
Drosophila melanogaster | ? | - |
? | |
NPLP4 protein | - |
Drosophila melanogaster | ? | - |
? | |
rho-7 protein | - |
Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DromeQC | isoform | Drosophila melanogaster |
glutaminyl cyclase | - |
Drosophila melanogaster |
isoDromeQC | isoform | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
isoform DromeQC | Drosophila melanogaster |
7.5 | 8 | isoform isoDromeQC | Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001013 | - |
PBD150 | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
0.0001093 | - |
PBD150 | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
0.0003706 | - |
PBD150 | isoform isoDromeQC, at 30°C in 50 mM MES (pH 6.0) | Drosophila melanogaster | |
0.000944 | - |
PBD150 | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
0.00248 | - |
PBD150 | isoform DromeQC, at 30°C in 50 mM MES (pH 6.0) | Drosophila melanogaster | |
0.003139 | - |
PBD150 | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
NPLP4 protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
4.6 | - |
NPLP4 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
6 | - |
NPLP4 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
7.4 | - |
NPLP4 protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
7.8 | - |
ALDH2 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
8.3 | - |
ALDH2 protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
13.9 | - |
DIM-10 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
15.9 | - |
39SrpL21 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
17.5 | - |
DIM-10 protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
20.1 | - |
NPLP4 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
20.2 | - |
39SrpL21 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
20.9 | - |
NPLP4 protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
20.9 | - |
39SrpL21 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
21.1 | - |
39SrpL21 protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
21.5 | - |
NPLP4 protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
21.9 | - |
39SrpL21 protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
22 | - |
MCCB protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
22.8 | - |
MCCB protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
23.8 | - |
NPLP4 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
25 | - |
MCCB protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
25.5 | - |
MCCB protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
25.9 | - |
DIM-10 protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
26.1 | - |
MCCB protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
26.2 | - |
rho-7 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
26.2 | - |
ALDH2 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
27.6 | - |
39SrpL21 protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
28.9 | - |
MCCB protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
30.2 | - |
rho-7 protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
31.2 | - |
DIM-10 protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
31.2 | - |
DIM-10 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
32 | - |
ALDH2 protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
32.3 | - |
MCCB protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
33.2 | - |
MCCB protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
33.9 | - |
ALDH2 protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
35 | - |
Acp33A protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
36 | - |
Acp33A protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
36.9 | - |
Acp33A protein | isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
38 | - |
Acp33A protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
40.9 | - |
ALDH2 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
40.9 | - |
rho-7 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
43.3 | - |
DIM-10 protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
43.4 | - |
rho-7 protein | isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
45.3 | - |
39SrpL21 protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
49.9 | - |
39SrpL21 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
50.2 | - |
rho-7 protein | isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
50.7 | - |
DIM-10 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
53.3 | - |
rho-7 protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
55 | - |
rho-7 protein | isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
59.3 | - |
DIM-10 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) | Drosophila melanogaster | |
69 | - |
ALDH2 protein | isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
73.1 | - |
ALDH2 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster | |
88 | - |
rho-7 protein | isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) | Drosophila melanogaster |