Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C367S | mutation within RING finger domain, mutant protein does not display significant ubiquitin ligase activity | Arabidopsis thaliana |
H348Y | mutation within RING finger domain, mutant protein does not display significant ubiquitin ligase activity | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SYH3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | expression in all developmental stages | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-ubiquitinyl-[UbcH5b]-L-cysteine + [NERF]-L-lysine | - |
Arabidopsis thaliana | [UbcH5b]-L-cysteine + N6-ubiquitinyl-[NERF]-L-lysine | autoubiquitylation reaction | ? |
Synonyms | Comment | Organism |
---|---|---|
At1g54150 | - |
Arabidopsis thaliana |
NERF | - |
Arabidopsis thaliana |
NFYA5 enhancing RING FINGER | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression is induced by drought stress | up |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform NERF is the cis-natural antisense transcript gene of NFYA5. NERF can produce siRNAs from their overlapping region and affect NFYA5 transcripts by functioning together with miR169. The NERF protein functions as an E3 ligase for ubiquitination. Overexpression of NERF or overlapping region cDNA leads to siRNA-NERF accumulation, miR169 repression, and NFYA5 transcript enhancement. Knockdown of NERF transcripts by an artificial miRNA enhances miR169 abundance and reduces NFYA5 transcripts | Arabidopsis thaliana |