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Literature summary for 2.3.2.27 extracted from

  • Li, W.; Tu, D.; Brunger, A.; Ye, Y.
    A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate (2007), Nature, 446, 333-337.
    View publication on PubMed
Search for more literature for 2.3.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKV5 isoform gp78
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 [gp78-ubiquitin-carrier protein Ub2g2]-S-ubiquitinyl-L-cysteine use of mouse Ube2g2 ubiquitin conjugating enzyme. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine residue C89 of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate Homo sapiens [gp78-ubiquitin-carrier protein Ub2g2]-S-[ubiquitinyl-N6-ubiquitinyl-L-lysine46]-L-cysteine
-
 ?
[gp78-ubiquitin-carrier protein Ub2g2]-S-(ubiquitin)x-L-cysteine + HERP-L-lysine HERP, ER-associated, short-lived protein that interacts with several E3 enzymes. Use of mouse Ube2g2 ubiquitin conjugating enzyme. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate Homo sapiens [gp78-ubiquitin-carrier protein Ub2g2]-L-cysteine + HERP-N6-(ubiquitin)x-L-lysine
-
 ?
[gp78-ubiquitin-carrier protein Ub2g2]-S-[ubiquitinyl-N6-ubiquitinyl-L-lysine46]-L-cysteine + x-2 [gp78-ubiquitin-carrier protein Ub2g2]-S-ubiquitinyl-L-cysteine
-
 Homo sapiens [gp78-ubiquitin-carrier protein Ub2g2]-S-(ubiquitin)x-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
AMFR
-
 Homo sapiens
gp78
-
 Homo sapiens
RNF45
-
 Homo sapiens