Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.27 extracted from

  • Du, Q.; Cui, W.; Zhang, C.; Yu, D.
    GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in soybean (Glycine max) (2010), Mol. Biol. Rep., 37, 685-693.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Glycine max

Protein Variants

Protein Variants Comment Organism
C194S mutation in highly conserved residue, loss of ubiqitin ligase activity Glycine max
C197S mutation in highly conserved residue, loss of ubiqitin ligase activity Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the protein contains a RING-H2 motif and an N-terminal transmembrane domain Glycine max 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43118
-
x * 43118, calculated Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max C6EUD3 isoform RFP1
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information transcript is detected in all organs analyzed Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[RFP1-ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [RFP1]-L-lysine in the presence of ubiquitin, ATP, rabbit E1, human E2 and recombinant isoform RFP1, accumulation of high molecular weight ubiquitinated RFP1 is detected, indicating its ability to autoubiquitinate Glycine max [RFP1-ubiquitin-carrier protein E2]-L-cysteine + [RFP1]-N6-ubiquitinyl-L-lysine
-
?

Subunits

Subunits Comment Organism
? x * 43118, calculated Glycine max

Synonyms

Synonyms Comment Organism
RFP1
-
Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max calculated
-
6.8

Expression

Organism Comment Expression
Glycine max trancription is downregulated by cold and drought treatments down
Glycine max trancription is upregulated by abscisic acid and salt stress up