Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli (BL21) as a fusion protein with maltose-binding protein MBP | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Capsicum annuum | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20570 | - |
calculated | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | G0T3B3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant fusion protein CaRING1-maltose-binding protein MBP, cell lysis, affinity chromatography of the the soluble fraction | Capsicum annuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression and ubiquitination of CaRING1 are required to induce cell death effectively in pepper leaves as a pathogen infection response | Capsicum annuum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP are not displayed in the absence of E1, E2, E3, ATP or ubiquitin | Capsicum annuum | ? | ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP is detected using anti-ubiquitin and anti-MBP antibody | ? |
Synonyms | Comment | Organism |
---|---|---|
CaRING1 | gene name, the RING domain belongs to the C3H2C3-type RING-H2 group which are essential for E3 ubiquitin ligase activity in the ubiquitin/26S proteasome system. The complete RING domain is essentiel for ubiquitination activity | Capsicum annuum |
E3 ubiquitin ligase RING1 | - |
Capsicum annuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Capsicum annuum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Capsicum annuum | calculated | - |
8.86 |
Organism | Comment | Expression |
---|---|---|
Capsicum annuum | expression is induced by avirulent Xanthomonas campestris pv vesicatoria infection | up |
General Information | Comment | Organism |
---|---|---|
physiological function | essential for hypersensitive response (HR) production. The CaRING1 expression triggers the hypersensitive cell death response. Transgenic Arabidopsis plants overexpressing CaRING1 also exhibit enhanced bacterial and fungal disease resistance, accompanied by the induction of salicylic acid against pathogen infection | Capsicum annuum |