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Literature summary for 2.3.2.26 extracted from

  • Osmundson, E.C.; Ray, D.; Moore, F.E.; Gao, Q.; Thomsen, G.H.; Kiyokawa, H.
    The HECT E3 ligase Smurf2 is required for Mad2-dependent spindle assembly checkpoint (2008), J. Cell Biol., 183, 267-277.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
C716A mutation in catalytic cysteine. Cells transfected with C716A fail to accumulate securin with significant reduction in Mad2 level. Cell show a significant increase in cells displaying misaligned chromosomes, lagging chromosomes during mitotic exit, and multinucleation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
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U2-OS cell
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 Homo sapiens
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General Information

General Information Comment Organism
physiological function ubiquitin ligase Smurf2 is required for the spindle checkpoint. Smurf2 localizes to the centrosome, mitotic midbody, and centromeres. Smurf2 depletion or the expression of a catalytically inactive Smurf2 results in misaligned and lagging chromosomes, premature anaphase onset, and defective cytokinesis. Smurf2 inactivation prevents nocodazole-treated cells from accumulating cyclin B and securin and prometaphase arrest. The silencing of Cdc20 in Smurf2-depleted cells restores mitotic accumulation of cyclin B and securin. Smurf2 depletion results in enhanced polyubiquitination and degradation of Mad2, a critical checkpoint effector. Mad2 is mislocalized in Smurf2-depleted cells Homo sapiens