Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.24 extracted from

  • Shin, D.Y.; Lee, H.; Park, E.S.; Yoo, Y.J.
    Assembly of different length of polyubiquitins on the catalytic cysteine of E2 enzymes without E3 ligase; a novel application of non-reduced/reduced 2-dimensional electrophoresis (2011), FEBS Lett., 585, 3959-3963.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Homo sapiens O00762
-
-
Homo sapiens P60604
-
-
Homo sapiens P61086
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information E3-independent ubiquitination by Ube2K produces unanchored but also Ube2K-linked polyubiquitins through thioester and isopeptide bonds. E3-independent assembly of polyubiquitins on the catalytic cysteine of Ube2K strongly supports the possibility of en bloc transfer for polyubiquitination. Different lengths of polyubiquitins are linked to different E2s through thioester bond. In vitro E3-independent ubiquitination by Ube2K simultaneously produces unanchored, isopeptde-linked, and thioester-linked polyubiquitin chains Homo sapiens ?
-
?
additional information isoform UbcH10 does not preassemble polyubiquitin chains on its acitve site cysteine Homo sapiens ?
-
?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + S-(ubiquitin)n-Ube2g2-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-(ubiquitin)(n+1)-Ube2g2-L-cysteine short chains of polyubiquitins can be linked bonds to E2 enzyme Ube2g2 ?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + S-(ubiquitin)n-Ube2K-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-(ubiquitin)(n+1)-Ube2K-L-cysteine longer chains of polyubiquitins can be linked bonds to E2 enzyme Ube2K. Ubiquitin chains are assembled on the active site cysteine of Ube2K through thioester bond ?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + UbcH10-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-UbcH10-L-cysteine
-
?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + Ube2g2-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-Ube2g2-L-cysteine
-
?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + Ube2K-L-cysteine
-
Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-Ube2K-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
UbcH10
-
Homo sapiens
UBE2C
-
Homo sapiens
UBE2G2
-
Homo sapiens
UBE2K
-
Homo sapiens