Protein Variants | Comment | Organism |
---|---|---|
S463D | complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 | Escherichia coli |
S463K | complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 | Escherichia coli |
S463T | 40% decrease in ratio kcat/KM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 9.0, 37°C | Escherichia coli | |
0.058 | - |
5-L-glutamyl-4-nitroanilide | mutant S463T, pH 9.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P18956 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
mutant S463T, pH 9.0, 37°C | Escherichia coli |
6.8 | - |
wild-type, pH 9.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
5-L-glutamyl-4-nitroanilide | mutant S463T, pH 9.0, 37°C | Escherichia coli | |
0.62 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 9.0, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
5-L-glutamyl-4-nitroanilide | mutant S463T, pH 9.0, 37°C | Escherichia coli | |
0.014 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 9.0, 37°C | Escherichia coli |