Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Lyu, R.C.; Hu, H.Y.; Kuo, L.Y.; Lo, H.F.; Ong, P.L.; Chang, H.P.; Lin, L.L.
    Role of the conserved Thr399 and Thr417 residues of Bacillus licheniformis gamma-Glutamyltranspeptidase as evaluated by mutational analysis (2009), Curr. Microbiol., 59, 101-106.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T399A complete loss of enzymatic activity Bacillus licheniformis
T399C complete loss of enzymatic activity Bacillus licheniformis
T399S dramatic reduction in activity Bacillus licheniformis
T399S precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme Bacillus licheniformis
T417A complete loss of enzymatic activity Bacillus licheniformis
T417A precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme, processed enzyme lacks activity Bacillus licheniformis
T417K complete loss of enzymatic activity Bacillus licheniformis
T417S dramatic reduction in activity Bacillus licheniformis
T417S precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis
0.09
-
5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
0.11
-
5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.25
-
mutant T399S, pH 8.0, 40°C Bacillus licheniformis
12.7
-
mutant T417S, pH 8.0, 40°C Bacillus licheniformis
83.7
-
wild-type, pH 8.0, 40°C Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.42
-
5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis
24.24
-
5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
109.5
-
5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13
-
5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis
269.5
-
5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
1504
-
5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis