Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Vergauwen, B.; Dudycz, L.W.; Dansercoer, A.; Devreese, B.
    A direct spectrophotometric gamma-glutamyltransferase inhibitor screening assay targeting the hydrolysis-only mode (2009), Biochem. Biophys. Res. Commun., 380, 591-596.
    View publication on PubMed
Search for more literature for 2.3.2.2

Application

Application Comment Organism
analysis simple, robust and highly sensitive assay based on an alpha-phenylthio-containing glutathione peptide mimic gamma-glutamyl-D,L-phenylthioglycylglycine that eliminates thiophenol upon GGT-catalyzed hydrolysis of the c-glutamyl peptide bond Equus caballus

Inhibitors

Inhibitors Comment Organism Structure
glutathione disulfide
-
 Equus caballus
S-(4-nitrobenzyl)glutathione
-
 Equus caballus
S-hexylglutathione
-
 Equus caballus
S-Methylglutathione
-
 Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.3
-
 gamma-glutamyl-D,L-phenylthioglycylglycine pH 7.5, 22°C Equus caballus

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Equus caballus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-glutamyl-D,L-phenylthioglycylglycine + acceptor
-
 Equus caballus gamma-glutamyl-acceptor + alpha-phenylthioglycyl-glycine
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00774
-
 S-(4-nitrobenzyl)glutathione pH 7.5, 22°C Equus caballus
0.0079
-
 S-Methylglutathione pH 7.5, 22°C Equus caballus
0.00865
-
 glutathione disulfide pH 7.5, 22°C Equus caballus
0.0103
-
 S-hexylglutathione pH 7.5, 22°C Equus caballus