Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can catalyze the transfer of L-glutamine to Gly-Gly to synthesize Gln-Gly-Gly, which is promising for the synthesis of valuable gamma-glutamyl peptides | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 32000 + 1 * 43000, SDS-PAGE | Bacillus subtilis |
43000 | - |
1 * 32000 + 1 * 43000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis NX-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73.36 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Arg | 113.6% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | 113.6% of the activity with Gly-Gly | Bacillus subtilis NX-2 | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Asn | 39.3% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Asn | - |
? | |
5-L-glutamyl-4-nitroanilide + Asn | 39.3% of the activity with Gly-Gly | Bacillus subtilis NX-2 | 4-nitroaniline + 5-L-glutamyl-Asn | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 4.5% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 4.5% of the activity with Gly-Gly | Bacillus subtilis NX-2 | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys | 46.9% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + DL-Phe | 39.9% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-DL-Phe | - |
? | |
5-L-glutamyl-4-nitroanilide + Glu | 4.4% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 33.2% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 33.2% of the activity with Gly-Gly | Bacillus subtilis NX-2 | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus subtilis NX-2 | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + His | 82.9% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-His | - |
? | |
5-L-glutamyl-4-nitroanilide + Ile | 20.7% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Ile | - |
? | |
5-L-glutamyl-4-nitroanilide + L-tyrosine | 24.1% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-tyrosine | - |
? | |
5-L-glutamyl-4-nitroanilide + Leu | 21.3% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Leu | - |
? | |
5-L-glutamyl-4-nitroanilide + Lys | 79.4% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Lys | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | 48.8% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + Pro | 13.8% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Pro | - |
? | |
5-L-glutamyl-4-nitroanilide + Ser | 12.8% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Ser | - |
? | |
5-L-glutamyl-4-nitroanilide + Thr | 27.3% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Thr | - |
? | |
5-L-glutamyl-4-nitroanilide + Tyr | 34.0% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Tyr | - |
? | |
5-L-glutamyl-4-nitroanilide + Val | 30.2% of the activity with Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Val | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 32000 + 1 * 43000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 70% of maximal activity, 60°C: about 90% of maximal activity, 70°C: about 40% of maximal activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 40% of maximal activity, pH 9.0: 65% of maximal activity | Bacillus subtilis |