Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Maleate | - |
Lepidochelys kempii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | no inhibition | Lepidochelys kempii | |
N-ethylmaleimide | no inhibition | Lepidochelys kempii | |
p-chloromercuribenzoate | no inhibition | Lepidochelys kempii | |
tosyl fluoride | inhibition by tosyl fluoride only in presence of maleate | Lepidochelys kempii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of Rathke's gland | Lepidochelys kempii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 54000 + x * 21000, SDS-PAGE | Lepidochelys kempii |
54000 | - |
x * 54000 + x * 21000, SDS-PAGE | Lepidochelys kempii |
200000 | - |
gel filtration | Lepidochelys kempii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lepidochelys kempii | - |
Kemp's ridley sea-turtle | - |
Purification (Comment) | Organism |
---|---|
from secretion | Lepidochelys kempii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gland | secretions of Rathke's gland | Lepidochelys kempii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2213 | - |
purified enzyme | Lepidochelys kempii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Lepidochelys kempii | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | best acceptors: free amino acids | Lepidochelys kempii | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Lepidochelys kempii | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Lepidochelys kempii | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-L-alanine + 5-L-glutamyl-L-alanine | shows also hydrolase activity with this substrate | Lepidochelys kempii | L-glutamine + 5-L-glutamyl-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-L-lysine + acceptor | shows also hydrolase activity with this substrate | Lepidochelys kempii | L-lysine + 5-L-glutamyl-acceptor | - |
? | |
glutathione + acceptor | - |
Lepidochelys kempii | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
S-methylglutathione + acceptor | shows also hydrolase activity with this substrate | Lepidochelys kempii | S-methyl-L-cysteinylglycine + 5-L-glutamyl-acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000 + x * 21000, SDS-PAGE | Lepidochelys kempii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lepidochelys kempii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Lepidochelys kempii |