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Literature summary for 2.3.2.2 extracted from

  • Goore, M.Y.; Thompson, J.F.
    gamma-Glutamyl transpeptidase from kidney bean fruit. I. Purification and mechanism of action (1967), Biochim. Biophys. Acta, 132, 15-26.
    View publication on PubMed
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
carboxylic acids
-
 Phaseolus vulgaris

General Stability

General Stability Organism
freezing or lyophilization inactivates purified enzyme Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition by diverse amino acids, overview Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 gel filtration Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phaseolus vulgaris

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Phaseolus vulgaris
a

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
 purified enzyme Phaseolus vulgaris

Storage Stability

Storage Stability Organism
0°C, at least 3 weeks Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Phaseolus vulgaris peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Phaseolus vulgaris peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Phaseolus vulgaris peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamylaniline + 5-L-glutamylaniline
-
 Phaseolus vulgaris aniline + 5-L-glutamyl-5-L-glutamylaniline
-
 ir
5-L-glutamylaniline + H2O
-
 Phaseolus vulgaris aniline + L-glutamic acid
-
 ir
5-L-glutamylaniline + S-methyl-L-cysteine
-
 Phaseolus vulgaris aniline + 5-L-glutamyl-S-methyl-L-cysteine
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-dependence of reaction kinetics Phaseolus vulgaris
9.5
-
-
 Phaseolus vulgaris