Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | effect of amino acids on activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | weak | Homo sapiens | |
glutathione | - |
Homo sapiens | |
iodoacetate | weak | Homo sapiens | |
Maleate | - |
Homo sapiens | |
additional information | inhibition by diverse amino acids, overview | Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens | |
NH4+ | weak | Homo sapiens | |
p-hydroxymercuribenzoate | weak | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-Glu-4-nitroanilide | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no effect | Homo sapiens | |
K+ | no effect | Homo sapiens | |
Li+ | no effect | Homo sapiens | |
Mg2+ | no effect | Homo sapiens | |
additional information | no activation by Zn2+ and Mn2+ | Homo sapiens | |
Na+ | no effect | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Homo sapiens |
additional information | - |
in the presence of Triton X-100: molecular aggregation | Homo sapiens |
21000 | - |
1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
57000 | - |
1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
90000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200.4 | - |
purified enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, t1/2: 2 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
glutathione + H2O | hydrolase reaction, concurrent to (auto-)transpeptidation | Homo sapiens | L-cysteinylglycine + L-glutamate | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.5 | Homo sapiens |
8 | - |
assay at | Homo sapiens |
8.2 | - |
- |
Homo sapiens |