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Literature summary for 2.3.2.2 extracted from

  • Karkowsky, A.M.; Bergamini, M.V.W.; Orlowski, M.
    Kinetic studies of sheep kidney gamma-glutamyl transpeptidase (1976), J. Biol. Chem., 251, 4736-4743.
    View publication on PubMed
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
Maleate 1.6fold Ovis aries
additional information no effect by phthalate, pyruvate and fumarate Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Ovis aries
Ca2+ no inhibition Ovis aries
Co2+ not affected Ovis aries
EDTA weak Ovis aries
iodoacetamide
-
 Ovis aries
iodoacetate weak Ovis aries
Mg2+ no inhibition Ovis aries
additional information not affected by Ni2+, K+, phtalate, pyruvate and fumarate Ovis aries
N-ethylmaleimide
-
 Ovis aries
Na+ not affected Ovis aries
p-chloromercuribenzoate
-
 Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Ovis aries
2.9
-
 L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate hydrolase reaction Ovis aries

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Ovis aries
K+ no effect Ovis aries
Mg2+ no effect Ovis aries
additional information not affected by Co2+ and Ni2+ Ovis aries
Na+ no effect Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Ovis aries
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ovis aries
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + H2O hydrolase reaction Ovis aries 4-nitroaniline + L-glutamate
-
 ir
glutathione + glutathione autotranspeptidation Ovis aries L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Ovis aries L-cysteinylglycine + L-glutamate
-
 ?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor acceptor specificity, overview Ovis aries L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
 ?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor hydrolysis in the absence of acceptor, methyl group at alpha-C prevents autotranspeptidation Ovis aries L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ovis aries