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Literature summary for 2.3.2.2 extracted from

  • Tarachand, U.
    Purification and properties of gamma-glutamyl transpeptidase from rat deciduoma (1984), J. Appl. Biochem., 6, 278-288.
    View publication on PubMed
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus
Ca2+ weak Rattus norvegicus
Co2+ weak Rattus norvegicus
EDTA weak Rattus norvegicus
Hg2+
-
 Rattus norvegicus
L- or D-Serine/borate L-serine/borate, not D-serine/borate Rattus norvegicus
L-serine
-
 Rattus norvegicus
Mg2+ no inhibition Rattus norvegicus
Mn2+ weak Rattus norvegicus
N-ethylmaleimide weak Rattus norvegicus
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 L-Glu-4-nitroanilide
-
 Rattus norvegicus
7.6
-
 glycylglycine
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 45000 + 1 * 23000, SDS-PAGE Rattus norvegicus
45000
-
 1 * 45000 + 1 * 23000, SDS-PAGE Rattus norvegicus
70000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar, female
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein binding of concanavalin A Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
papain Rattus norvegicus
Triton X-100 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
deciduoma cell
-
 Rattus norvegicus
-
uterus
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in subcellular fractions Rattus norvegicus
104.5
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, at least 30 days without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycine glutamyl transfer at 17.2% Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine best acceptor Rattus norvegicus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-alanine glutamyl transfer at 32% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-arginine glutamyl transfer at 30% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
5-L-glutamyl-4-nitroanilide + L-aspartate glutamyl transfer at 8.6% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine glutamyl transfer at 43% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + L-histidine glutamyl transfer at 8.6% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline glutamyl transfer at 17% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
-
 ?
5-L-glutamyl-4-nitroanilide + L-isoleucine glutamyl transfer at 17% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-leucine glutamyl transfer at 20% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-lysine glutamyl transfer at 26% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine glutamyl transfer at 39% the rate with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
5-L-glutamyl-4-nitroanilide + L-phenylalanine glutamyl transfer at 26% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-serine glutamyl transfer at 28% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
5-L-glutamyl-4-nitroanilide + L-threonine glutamyl transfer at 20% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
5-L-glutamyl-4-nitroanilide + L-valine glutamyl transfer at 20% the rate of the reaction with glycylglycine Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 45000 + 1 * 23000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
 t1/2: 5 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.022
-
 L-serine in presence of borate Rattus norvegicus