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Literature summary for 2.3.2.15 extracted from
- Cadmium(II)-stimulated enzyme activation of Arabidopsis thaliana phytochelatin synthase 1 (2011), J. Inorg. Biochem., 105, 111-117.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GSH | the maximal activity is attained at GSH concentrations around 10 and 15 mM when the enzyme is assayed in the presence of 0.005 and 0.01 mM total Cd2+, respectively | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Rosetta (DE3) pLysS cells | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | the enzyme also possesses a second Cd2+ binding site where Cd2+ binds to induce an inhibitory effect | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | the enzyme possesses two Cd2+ binding sites, Cd2+ binds to one of these sites to activate the enzyme. In the presence of 0.01 mM Cd2+, activity increases and reaches the maximum rate with an increase in GSH concentration up to 15 mM | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56500 | - |
x * 56500, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity column chromatography and HisTrap HP column chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56500, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-glutamylcysteinyl transpeptidase | - |
Arabidopsis thaliana |
| PCS1 | - |
Arabidopsis thaliana |
| phytochelatin synthase | - |
Arabidopsis thaliana |
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