Literature summary for 2.3.2.13 extracted from

Steffen, W.; Ko, F.C.; Patel, J.; Lyamichev, V.; Albert, T.J.; Benz, J.; Rudolph, M.G.; Bergmann, F.; Streidl, T.; Kratzsch, P.; Boenitz-Dulat, M.; Oelschlaegel, T.; Schraeml, M.
Discovery of a microbial transglutaminase enabling highly site-specific labeling of proteins (2017), J. Biol. Chem., 292, 15622-15635 .

Application

Application	Comment	Organism
synthesis	the Kutzneria albida microbial transglutaminase enabling highly site-specific labeling of proteins. Its site specificity, favorable kinetics, ease of use, and cost-effective production render KalbTG an attractive tool for a broad range of applications, including production of therapeutic antibody-drug conjugates. The high activity and low molecular mass of KalbTG signifies a key advantage for mass production and enzymatic labeling purposes	Kutzneria albida

Cloned(Commentary)

Cloned (Comment)	Organism
functional recombinant expression of N-terminally His-tagged soluble MTG in Escherichia coli strain Bl21 Tuner	Kutzneria albida

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant soluble KalbTG is crystallized at 22°C using the sitting drop vapor diffusion method by 1:1 mixing of 200 nl of 8 mg/ml protein in PBS with an unbuffered reservoir consisting of 0.2 M ammonium tartrate, and 20% PEG 3350, X-ray diffraction structure determination and analysis at 1.9 A resolution, structure modeling, the first 19 N-terminal amino acids (MGGGSTTAQAAAVAAPTPR) and the C-terminal artificial GGGSHis8 tag are disordered in the structure	Kutzneria albida

Crystallization (Comment)

Organism

purified recombinant soluble KalbTG is crystallized at 22°C using the sitting drop vapor diffusion method by 1:1 mixing of 200 nl of 8 mg/ml protein in PBS with an unbuffered reservoir consisting of 0.2 M ammonium tartrate, and 20% PEG 3350, X-ray diffraction structure determination and analysis at 1.9 A resolution, structure modeling, the first 19 N-terminal amino acids (MGGGSTTAQAAAVAAPTPR) and the C-terminal artificial GGGSHis8 tag are disordered in the structure

Kutzneria albida

Protein Variants

Protein Variants	Comment	Organism
additional information	by first reacting a bifunctionalized peptide with the more specific KalbTG and in a second step with the less specific MTG from Streptomyces mobaraensis (UniProt ID P81453), a successful bio-orthogonal labeling system is demonstrated. Fusing the KalbTG recognition motif to an antibody allows for site-specific and ratio-controlled labeling using low label excess	Kutzneria albida

Inhibitors

Inhibitors	Comment	Organism	Structure
ammonium	a natural inhibitor of the enzyme, prevents cross-linking activity	Kutzneria albida
N-(biotinyl)cadaverine	-	Kutzneria albida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protein glutamine + alkylamine	Kutzneria albida	-	protein N5-alkylglutamine + NH3	-	?
protein glutamine + alkylamine	Kutzneria albida DSM 43870	-	protein N5-alkylglutamine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Kutzneria albida	W5WHY8	-	-
Kutzneria albida DSM 43870	W5WHY8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	microbial transglutaminase, MTG, from Kutzneria albida (KalbTG) exhibits no cross-reactivity with known MTG substrates or commonly used target proteins, such as antibodies. The motifs YRYRQ and RYESK are the best Gln and Lys substrates of KalbTG, respectively. Substrate specificity analysis, overview	Kutzneria albida	?	-	-
additional information	microbial transglutaminase, MTG, from Kutzneria albida (KalbTG) exhibits no cross-reactivity with known MTG substrates or commonly used target proteins, such as antibodies. The motifs YRYRQ and RYESK are the best Gln and Lys substrates of KalbTG, respectively. Substrate specificity analysis, overview	Kutzneria albida DSM 43870	?	-	-
protein glutamine + alkylamine	-	Kutzneria albida	protein N5-alkylglutamine + NH3	-	?
protein glutamine + alkylamine	-	Kutzneria albida DSM 43870	protein N5-alkylglutamine + NH3	-	?

Synonyms

Synonyms	Comment	Organism
KalbTG	-	Kutzneria albida
KALB_7456	-	Kutzneria albida
microbial transglutaminases	-	Kutzneria albida
MTG	-	Kutzneria albida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	42	assay at	Kutzneria albida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Kutzneria albida