Literature summary for 2.3.2.13 extracted from

Yu, C.M.; Zhou, H.; Zhang, W.F.; Yang, H.M.; Tang, J.B.
Site-specific, covalent immobilization of BirA by microbial transglutaminase A reusable biocatalyst for invitro biotinylation (2016), Anal. Biochem., 511, 10-12 .

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Application

Application	Comment	Organism
biotechnology	use of MTG to site-specifically and covalently immobilize a substrate peptide-tagged protein, e.g. BirA, to a support, i.e. amine-modified magnetic microspheres (MMS)	Streptomyces mobaraensis

Protein Variants

Protein Variants	Comment	Organism
additional information	development and evaluation of a method for production of a reusable immobilized recombinant His-tagged Escherichia coli biotin ligase (BirA) onto amine-modified magnetic microspheres (MMS) via covalent cross-linking catalyzed using microbial transglutaminase (MTG). The site-specifically immobilized BirA exhibited approximately 95% of enzymatic activity of the free BirA, and without a significant loss in intrinsic activity after 10 rounds of recycling. Method, overview	Streptomyces mobaraensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces mobaraensis	P81453	-	-

Synonyms

Synonyms	Comment	Organism
microbial transglutaminase	-	Streptomyces mobaraensis
MTG	-	Streptomyces mobaraensis

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Release 2023.1 (January 2023)