Cloned (Comment) | Organism |
---|---|
the pro-enzyme is expressed in Yarrowia lipolytica Po1g or Po1h strains | Streptomyces hygroscopicus |
Protein Variants | Comment | Organism |
---|---|---|
N160Q | the mutant shows wild type activity | Streptomyces hygroscopicus |
N160Q/N355Q | the mutant shows 57% activity compared to the wild type enzyme | Streptomyces hygroscopicus |
N355Q | the mutant shows 147% activity compared to the wild type enzyme | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
N-Cbz-Gln-Gly | mutant enzyme N160Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
48.4 | - |
N-Cbz-Gln-Gly | mutant enzyme N160Q/N355Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
54.7 | - |
N-Cbz-Gln-Gly | mutant enzyme N355Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
59.5 | - |
N-Cbz-Gln-Gly | wild type enzyme, at pH 6.0 and 37°C | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43600 | - |
x * 43600, pro-enzyme, calculated from amino acid sequence | Streptomyces hygroscopicus |
45000 | - |
x * 45000, pro-enzyme, SDS-PAGE | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-L-glutamine + alkylamine | Streptomyces hygroscopicus | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
[protein]-L-glutamine + alkylamine | Streptomyces hygroscopicus WSH03-13 | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | D9J009 | - |
- |
Streptomyces hygroscopicus WSH03-13 | D9J009 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Streptomyces hygroscopicus |
Purification (Comment) | Organism |
---|---|
Fractogel EMD SO3- column chromatography | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Cbz-Gln-Gly + alkylamine | - |
Streptomyces hygroscopicus | ? + NH3 | - |
? | |
N-Cbz-Gln-Gly + alkylamine | - |
Streptomyces hygroscopicus WSH03-13 | ? + NH3 | - |
? | |
[protein]-L-glutamine + alkylamine | - |
Streptomyces hygroscopicus | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
[protein]-L-glutamine + alkylamine | - |
Streptomyces hygroscopicus WSH03-13 | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43600, pro-enzyme, calculated from amino acid sequence | Streptomyces hygroscopicus |
? | x * 45000, pro-enzyme, SDS-PAGE | Streptomyces hygroscopicus |
Synonyms | Comment | Organism |
---|---|---|
TGase | - |
Streptomyces hygroscopicus |
transglutaminase | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces hygroscopicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | the half-life of the recombinant wild type pro-enzyme at 37°C reaches 34 min. The zymogen retains approximately 80% of the activity after incubation at 20°C for 30 min and is approximately inactivated after the same time at 50°C | Streptomyces hygroscopicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.6 | - |
N-Cbz-Gln-Gly | mutant enzyme N160Q/N355Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
23.8 | - |
N-Cbz-Gln-Gly | mutant enzyme N355Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
25.3 | - |
N-Cbz-Gln-Gly | mutant enzyme N160Q, at pH 6.0 and 37°C | Streptomyces hygroscopicus | |
31.5 | - |
N-Cbz-Gln-Gly | wild type enzyme, at pH 6.0 and 37°C | Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Streptomyces hygroscopicus |