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Literature summary for 2.3.2.13 extracted from

  • Kanchan, K.; Erguelen, E.; Kiraly, R.; Simon-Vecsei, Z.; Fuxreiter, M.; Fesues, L.
    Identification of a specific one amino acid change in recombinant human transglutaminase 2 that regulates its activity and calcium sensitivity (2013), Biochem. J., 455, 261-272.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
G224V the mutation increases the enzyme's calcium-binding affinity and transamidation activity 10fold and isopeptidase activity severalfold Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
alpha2 plasmin inhibitor at pH 7.5 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-L-glutamine + alkylamine Homo sapiens
-
[protein]-N5-alkyl-L-glutamine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P21980
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-APE(gamma-Cad-2,4-dinitrophenyl)QEA + Gly-OMe
-
Homo sapiens ?
-
?
5-(biotinamido)pentylamine + N,N-dimethylated casein
-
Homo sapiens ? + NH3
-
?
alpha2 plasmin inhibitor + ?
-
Homo sapiens ?
-
?
[protein]-L-glutamine + alkylamine
-
Homo sapiens [protein]-N5-alkyl-L-glutamine + NH3
-
?

Synonyms

Synonyms Comment Organism
TG2
-
Homo sapiens
transglutaminase 2
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
melting temperature Homo sapiens