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Literature summary for 2.3.2.13 extracted from
- Purification and properties of transglutaminase from soybean (Glycine max) leaves (1996), Biochem. Biophys. Res. Commun., 223, 288-292.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 10 mM, approx. 1.8fold activation | Glycine max |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GTP | - |
Glycine max | |
| N-ethylmaleimide | 0.1 mM, 60% inhibition | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
spermidine | - |
Glycine max | |
| 0.069 | - |
spermine | - |
Glycine max | |
| 0.11 | - |
putrescine | - |
Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+ is not required for activity | Glycine max |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sepharose, Blue-Sepharose CL-6B, omega-aminohexyl agarose, alpha-casein agarose | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-bound gamma-glutamine + alkylamine | - |
Glycine max | protein N5-alkylglutamine + NH3 | - |
? | |
| putrescine + N,N'-dimethylcasein | spermine and spermidine can replace putrescine | Glycine max | ? | - |
? | |
| spermidine + N,N'-dimethylcasein | - |
Glycine max | ? | - |
? | |
| spermine + N,N'-dimethylcasein | - |
Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Glycine max |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
GTP | - |
Glycine max | |
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