Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | inhibition of tissue transglutaminase | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | Homo sapiens | involved in a wide variety of cellular processes, including growth, differentiation, stabilization of cytoskeleton | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | Homo sapiens | last enzyme in blood coagulation forming intermolecular gamma-glutamyl-epsilon-lysine crosslinks between fibrin molecules | protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cytosolic tissue transglutaminase, DE52, heparin-agarose, casein-agarose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-431 cell | epidermal carcinoma cell line | Homo sapiens | - |
epidermis | stratum corneum | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.156 | - |
tissue transglutaminase | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris, pH 7.5, 1 mM EDTA/0.5 mM DTT, up to 2 weeks, no loss of activity | Homo sapiens |
dilute enzyme solutions are unstable to storage at 4°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | involved in a wide variety of cellular processes, including growth, differentiation, stabilization of cytoskeleton | Homo sapiens | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | last enzyme in blood coagulation forming intermolecular gamma-glutamyl-epsilon-lysine crosslinks between fibrin molecules | Homo sapiens | protein N5-alkylglutamine + NH3 | - |
? | |
putrescine + N,N'-dimethylcasein | - |
Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
at least 20 min stable, pH 9.0 | Homo sapiens |
37 | - |
t1/2: 1-2 min, pH 9.0 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
t1/2: 1-2 min stable at 37°C, at least 20 min stable at 4°C | Homo sapiens |