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Literature summary for 2.3.2.12 extracted from

  • Triboulet, S.; Bougault, C.M.; Laguri, C.; Hugonnet, J.E.; Arthur, M.; Simorre, J.P.
    Acyl acceptor recognition by Enterococcus faecium L,D-transpeptidase Ldtfm (2015), Mol. Microbiol., 98, 90-100.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterococcus faecium

Protein Variants

Protein Variants Comment Organism
K372A the mutant shows 7% of wild type activity Enterococcus faecium
K372A/R437A the mutant shows 2% of wild type activity Enterococcus faecium
R437A the mutant shows 26% of wild type activity Enterococcus faecium
W425A the mutant shows 0.04% of wild type activity Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
ertapenem
-
 Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2 Enterococcus faecium the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide tRNA1 + peptidyl(aminoacyl-tRNA2)
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 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2 the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide Enterococcus faecium tRNA1 + peptidyl(aminoacyl-tRNA2)
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 ?

Synonyms

Synonyms Comment Organism
L,D-transpeptidase
-
 Enterococcus faecium
Ldtfm
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 Enterococcus faecium