Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecium |
Protein Variants | Comment | Organism |
---|---|---|
K372A | the mutant shows 7% of wild type activity | Enterococcus faecium |
K372A/R437A | the mutant shows 2% of wild type activity | Enterococcus faecium |
R437A | the mutant shows 26% of wild type activity | Enterococcus faecium |
W425A | the mutant shows 0.04% of wild type activity | Enterococcus faecium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ertapenem | - |
Enterococcus faecium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-tRNA1 + aminoacyl-tRNA2 | Enterococcus faecium | the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-tRNA1 + aminoacyl-tRNA2 | the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide | Enterococcus faecium | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L,D-transpeptidase | - |
Enterococcus faecium |
Ldtfm | - |
Enterococcus faecium |