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Literature summary for 2.3.2.12 extracted from

  • Thompson, J.; Kim, D.F.; O'Connor, M.; Lieberman, K.R.; Bayfield, M.A.; Gregory, S.T.; Green, R.; Noller, H.F.; Dahlberg, A.E.
    Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit (2001), Proc. Natl. Acad. Sci. USA, 98, 9002-9007.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
chloramphenicol weak peptide bond formation inhibition only by ribosomes with A2451C, A2451U or A2451G, peptide bond formation inhibition by ribosomes with G2447A is essentially unimpaired Geobacillus stearothermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome 50S subunit, wild type ribosomes, ribosomes with site-directed mutated residues: A2451, G2447 Geobacillus stearothermophilus 5840
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Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-AcMet-tRNA + puromycin
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Geobacillus stearothermophilus tRNA + N-AcMet-puromycin
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
enzyme assay Geobacillus stearothermophilus