Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anisomycin | inhibition of formation of AcPhe-puromycin catalyzed by rabbit reticulocyte ribosomes | Oryctolagus cuniculus | |
blasticidin S | inhibition of formation of Ac-Phe-puromycin catalyzed by rabbit reticulocyte ribosomes | Oryctolagus cuniculus | |
sparsomycin | inhibition of formation of AcPhe-puromycin catalyzed by rabbit reticulocyte ribosomes | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
ribosome | 80S ribosomes | Oryctolagus cuniculus | 5840 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | salt-washed ribosomes | Oryctolagus cuniculus | - |
reticulocyte | unwashed ribosomes | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AcPhe-tRNA + puromycin | AcPhe-tRNA-polyU-80S ribosome complex | Oryctolagus cuniculus | tRNA + AcPhe-puromycin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay, reaction in solution | Oryctolagus cuniculus |
37 | - |
enzyme assay, disk reaction | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
AcPhe-tRNA | reaction in solution | Oryctolagus cuniculus | |
0.0483 | - |
AcPhe-tRNA | disk reaction | Oryctolagus cuniculus |