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Literature summary for 2.3.2.11 extracted from

  • Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
    Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol (2009), Mol. Microbiol., 71, 551-565.
    View publication on PubMed
Search for more literature for 2.3.2.11

Cloned(Commentary)

Cloned (Comment) Organism
into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 determined by SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanyl-tRNAAla + phosphatidylglycerol Pseudomonas aeruginosa
-
 tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-tRNAAla + phosphatidylglycerol
-
 Pseudomonas aeruginosa tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
A-PG synthase
-
 Pseudomonas aeruginosa
A-PGS
-
 Pseudomonas aeruginosa
alanylphosphatidylglycerol synthase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 in vitro activity assay Pseudomonas aeruginosa
35
-
 in vivo activity assay Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 in vivo activity assay Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa gene expression is found significantly up-regulated under acidic conditions up