Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | slight increase in activity | Solanum lycopersicum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum lycopersicum |
Crystallization (Comment) | Organism |
---|---|
homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
H276Y | 58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
chlorogenic acid | pH 4.0, 30°C | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | enzyme catalyzes different reactions in different compartments | Solanum lycopersicum | 5737 | - |
vacuole | enzyme catalyzes different reactions in different compartments | Solanum lycopersicum | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slight increase in activity | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 chlorogenic acid | Solanum lycopersicum | reaction is irreversible | 1,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir | |
2 chlorogenic acid | Solanum lycopersicum | reaction is irreversible | 3,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir | |
2 chlorogenic acid | Solanum lycopersicum | reaction is irreversible | 4,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q70G32 | bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.3 | - |
pH 4.0, 30°C | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 chlorogenic acid | reaction is irreversible | Solanum lycopersicum | 1,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir | |
2 chlorogenic acid | reaction is irreversible | Solanum lycopersicum | 3,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir | |
2 chlorogenic acid | reaction is irreversible | Solanum lycopersicum | 4,5-dicaffeoylquinic acid + quinate | products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid | ir |
Synonyms | Comment | Organism |
---|---|---|
CCT | - |
Solanum lycopersicum |
chlorogenate:chlorogenate transferase | - |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Solanum lycopersicum |