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Literature summary for 2.3.1.97 extracted from

  • Seaton, K.; Smith, C.
    N-Myristoyltransferase isozymes exhibit differential specificity for human immunodeficiency virus type 1 Gag and Nef (2008), J. Gen. Virol., 89, 288-296.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Protein Variants

Protein Variants Comment Organism
additional information selectively depletion by siRNA of NMT1 and/or NMT2 from HEK-293T cells expressing a recombinant NefsgGFP fusion protein, NMT1 depletion has minimal effect on the intracellular distribution of Nef-sgGFP, whereas depletion of NMT2 alters distribution to a diffuse, widespread pattern, mimicking that of a myristoylation-deficient mutant of Nef-sgGFP, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
HIV1 Nef protein N-terminal peptide Nef peptide acts as a competitive inhibitor for the myristoylation of Gag; Nef peptide acts as a competitive inhibitor for the myristoylation of Gag Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information NMT1 and NMT2 have 30 and 130fold lower Km values for Nef than Gag, respectively. Michaelis-Menten kinetics, and kinetic modelling of isozymes, overview Homo sapiens
0.0015
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens
0.011
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens
0.196
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens
0.33
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetradecanoyl-CoA + glycyl-HIV1 Gag protein N-terminal peptide Homo sapiens
-
 CoA + N-tetradecanoyl-glycyl-HIV1 Gag protein N-terminal peptide
-
 ?
tetradecanoyl-CoA + glycyl-HIV1 Nef protein N-terminal peptide Homo sapiens
-
 CoA + N-tetradecanoyl-glycyl-HIV1 Nef protein N-terminal peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60551 isozyme NMT2; isozyme NMT2
-
Homo sapiens P30419 isozyme NMT1; isozyme NMT1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-myristoyltransferase isozymes exhibit differential specificity for human immunodeficiency virus type 1 Gag and Nef, overview Homo sapiens ?
-
 ?
myristoyl-CoA + HIV1 Gag protein N-terminal peptide
-
 Homo sapiens N-myristoyl-HIV1 Gag protein N-terminal peptide + CoA
-
 ?
myristoyl-CoA + HIV1 Nef protein N-terminal peptide
-
 Homo sapiens N-myristoyl-HIV1 Nef protein N-terminal peptide + CoA
-
 ?
tetradecanoyl-CoA + glycyl-HIV1 Gag protein N-terminal peptide
-
 Homo sapiens CoA + N-tetradecanoyl-glycyl-HIV1 Gag protein N-terminal peptide
-
 ?
tetradecanoyl-CoA + glycyl-HIV1 Gag protein N-terminal peptide i.e. myristoyl-CoA Homo sapiens CoA + N-tetradecanoyl-glycyl-HIV1 Gag protein N-terminal peptide
-
 ?
tetradecanoyl-CoA + glycyl-HIV1 Nef protein N-terminal peptide
-
 Homo sapiens CoA + N-tetradecanoyl-glycyl-HIV1 Nef protein N-terminal peptide
-
 ?
tetradecanoyl-CoA + glycyl-HIV1 Nef protein N-terminal peptide i.e. myristoyl-CoA, NMT2 has approximately 5fold higher catalytic efficiency for the myristoylation of Nef than NMT1 Homo sapiens CoA + N-tetradecanoyl-glycyl-HIV1 Nef protein N-terminal peptide
-
 ?

Synonyms

Synonyms Comment Organism
N-myristoyltransferase
-
 Homo sapiens
NMT
-
 Homo sapiens
NMT1
-
 Homo sapiens
NMT2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.032
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens
0.052
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens
0.107
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens
0.265
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information competitive inhibition kinetics of HIV1 Gag and Nef protein N-terminal peptides, overview Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.98
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens
1
-
 HIV1 Gag protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens
4.9
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT1 Homo sapiens
23.6
-
 HIV1 Nef protein N-terminal peptide pH 7.4, 37°C, isozyme NMT2 Homo sapiens