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Literature summary for 2.3.1.97 extracted from
- Recombinant bovine spleen myristoyl CoA: protein N-myristoyltransferase (1998), Mol. Cell. Biochem., 189, 91-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of expression plasmid encoding the enzyme, lacking the first 8 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, expression in Escherichia coli DH5alpha, cDNA from spleen | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
p60src-derived peptide | recombinant enzyme | Bos taurus | |
| 0.2 | - |
cAMP-dependent protein kinase-derived peptide | recombinant enzyme | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| spleen enzyme, recombinant from Escherichia coli | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.048 | - |
purified recombinant protein | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + cAMP-dependent protein kinase-derived peptide | - |
Bos taurus | N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA | - |
? |  |
| myristoyl-CoA + glycylpeptide | - |
Bos taurus | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + p60src-derived peptide | - |
Bos taurus | N-myristoylated p60src-derived peptide + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, tag cleaved off by enterokinase, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NMT | - |
Bos taurus |
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Release 2023.1 (January 2023)
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