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Literature summary for 2.3.1.97 extracted from

  • Raju, R.V.S.; Datla, R.S.S.; Sharma, R.K.
    Expression of human N-myristoyltransferase in Escherichia coli. Comparison with N-myristoyltransferases expressed in different tissues (1996), Mol. Cell. Biochem., 155, 69-76.
    View publication on PubMed
Search for more literature for 2.3.1.97

Activating Compound

Activating Compound Comment Organism Structure
acetonitrile 2.5fold activation at 5% v/v Homo sapiens
ethanol 5fold activation at 10% v/v Homo sapiens
SDS 3.2fold activation at 1.73 mM Homo sapiens
Tris activates about 2.5fold at 225 mM compared to 40 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
construction of expression plasmid encoding the enzyme, lacking the first 9 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, overexpression in Escherichia coli DH5alpha Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
factor NIP71 bovine brain inhibitor protein; strong inhibition in a concentration dependent manner, noncompetitive Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 1 * 49000, tag cleaved off by enterokinase, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
acetonitrile, stable in 5% v/v Homo sapiens
ethanol, stable in 10% v/v Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.248
-
 purified wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + cAMP-dependent protein kinase-derived peptide
-
 Homo sapiens N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA
-
 ?
myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg pp60src-derived peptide Homo sapiens N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA
-
 ?
myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Homo sapiens N-myristoylglycylpeptide + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, tag cleaved off by enterokinase, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NMT
-
 Homo sapiens