Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme forms multiple high molecular weight aggregates of fully active monomers | Bos taurus |
60000 | - |
1 * 60000, SDS-PAGE | Bos taurus |
126000 | 390000 | high molecular weight aggregates, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Bos taurus |
Purification (Comment) | Organism |
---|---|
multiple forms from brain | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-15°C, conversion of 60000 Da form into 47000 Da form, loss of 30% activity within 10 days | Bos taurus |
4°C, conversion of 60000 Da form into 47000 Da form, activity is stable over several months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg | pp60src-derived peptide | Bos taurus | N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg + CoA | - |
? | |
myristoyl-CoA + glycylpeptide | specific for Gly at N-terminus | Bos taurus | N-myristoylglycylpeptide + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Bos taurus |
monomer | 1 * 47000-49000, active form after storage at 4°C, SDS-PAGE | Bos taurus |
More | 2 interconvertable forms of 60000 and 47000 Da, both catalytically active, formation of multimeric complexes, molecular weight reduction is not only due to proteolysis but has a possible regulatory role in vivo | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
NMT | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bos taurus |