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Literature summary for 2.3.1.97 extracted from

  • Wiegand, R.C.; Carr, C.; Minnerly, J.C.; Pauley, A.M.; Carron, C.P.; Langner, C.A.; Duronio, R.J.; Gordon, J.I.
    The Candida albicans myristoyl-CoA:protein N-myristoyltransferase gene. Isolation and expression in Saccharomyces cerevisiae and Escherichia coli (1992), J. Biol. Chem., 267, 8591-8598.
    View publication on PubMed
Search for more literature for 2.3.1.97

Application

Application Comment Organism
medicine target for antiviral and antifungal therapy Candida albicans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, DNA sequence determination Candida albicans

Protein Variants

Protein Variants Comment Organism
additional information
-
 Candida albicans
additional information temperature sensitive mutant strain nmt1-181, exchange of Gly to Asp, 10fold increased Km for myristoyl-CoA at 36°C Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00023
-
 Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.0044
-
 Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser
-
 Saccharomyces cerevisiae
0.006
-
 Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Candida albicans
0.017
-
 Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser
-
 Candida albicans
0.03
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.6
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence, alignment Saccharomyces cerevisiae
additional information
-
 amino acid sequence, alignment Candida albicans
51880
-
 calculation from gene sequence Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myristoyl-CoA + glycylpeptide Saccharomyces cerevisiae
-
 N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide Candida albicans
-
 N-myristoylglycylpeptide + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
 single copy gene nmt1
-
Candida albicans
-
 strain ATCC 32354
-
Saccharomyces cerevisiae
-
 gene nmt1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, and recombinant enzyme from Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae
additional information
-
-
 Candida albicans
0.000024
-
 purified native enzyme Candida albicans
0.000149
-
 purified recombinant enzyme Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser
-
 Candida albicans N-myristoyl-Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Candida albicans N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Candida albicans N-myristoyl-Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser
-
 Saccharomyces cerevisiae N-myristoyl-Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser + CoA
-
 ?
myristoyl-CoA + glycylpeptide
-
 Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide
-
 Candida albicans N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide overview peptides and proteins Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide overview peptides and proteins Candida albicans N-myristoylglycylpeptide + CoA
-
 ?
palmitoyl-CoA + glycylpeptide no activity Candida albicans N-palmitoylglycylpeptide + CoA
-
 ?

Synonyms

Synonyms Comment Organism
NMT
-
 Saccharomyces cerevisiae
NMT
-
 Candida albicans