Cloned (Comment) | Organism |
---|---|
expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli | Arachis hypogaea |
Protein Variants | Comment | Organism |
---|---|---|
G374L | no condensing activity | Arachis hypogaea |
P375G | product profile of p-coumaroyl condensing reaction changed to: 42% p-coumaroyltriacetic acid, 27% resveratrol, 24% bisnoryangonin and 7.3% naringenin | Arachis hypogaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
malonyl-CoA | P375G mutant enzyme | Arachis hypogaea | |
0.002 | - |
malonyl-CoA | - |
Arachis hypogaea | |
0.016 | - |
p-Coumaroyl-CoA | P375G mutant enzyme | Arachis hypogaea | |
0.022 | - |
p-Coumaroyl-CoA | - |
Arachis hypogaea | |
0.022 | - |
malonyl-CoA | malonyl-CoA decarboxylation, P375G mutant enzyme | Arachis hypogaea | |
0.031 | - |
malonyl-CoA | malonyl-CoA decarboxylation | Arachis hypogaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant resveratrol synthase, Ni2+-iminodiacetic acid-Sepharose | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA | - |
Arachis hypogaea | 3,4',5-trihydroxystilbene + CoA + CO2 | 79% resveratrol, side products: 10% p-coumaroyltriacetic acid lactone, 8.6% bisnoryangonin and 1.7% naringenin | ? |