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Literature summary for 2.3.1.91 extracted from
- Purification and characterization of sinapine synthase from seeds of Brassica napus (1993), Arch. Biochem. Biophys., 300, 622-628.
General Stability
| General Stability | Organism |
|---|---|
| choline chloride stabilizes | Brassica napus |
| repeated freeze-thawing, stable to | Brassica napus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Brassica napus |  |
| Hg2+ | - |
Brassica napus | |
| additional information | no inhibition by IAA, NEM, tosylphenylalanine chloromethylketone, diethylchlorocarbamate, p-nitrophenylchloroformate | Brassica napus | |
| PMSF | - |
Brassica napus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for divalent cations | Brassica napus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition and sequence | Brassica napus |
| 28000 | - |
2 * 28000, SDS-PAGE | Brassica napus |
| 65000 | - |
gel filtration | Brassica napus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
cv. Westar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica napus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | immature dark-green | Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
purified enzyme | Brassica napus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, at least 2 months | Brassica napus |
| 4°C, 2 weeks | Brassica napus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-feruloyl-beta-D-glucose + choline | 40% as active as 1-sinapoylglucose | Brassica napus | D-glucose + feruloylcholine | - |
? | |
| 1-O-sinapoyl-beta-D-glucose + choline | no acceptors are betaine or CDP-choline | Brassica napus | D-glucose + sinapoylcholine | i.e. sinapine | ir | |
| 1-O-sinapoyl-beta-D-glucose + choline | specific for 1-O-sinapoyl-beta-glucose | Brassica napus | D-glucose + sinapoylcholine | i.e. sinapine | ir | |
| 1-p-coumaroyl-beta-D-glucose + choline | 25% | Brassica napus | D-glucose + p-coumaroylcholine | - |
? | |
| caffeoyl-beta-D-glucose + choline | poor substrate | Brassica napus | D-glucose + caffeoylcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28000, SDS-PAGE | Brassica napus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min stable | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.1 | Brassica napus |
| 6.6 | 7.2 | - |
Brassica napus |
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Release 2023.1 (January 2023)
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