Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong, above 5 mM | Raphanus sativus | |
Ca2+ | strong, above 5 mM | Sinapis alba | |
Co2+ | strong, above 5 mM | Raphanus sativus | |
Co2+ | strong, above 5 mM | Sinapis alba | |
dithioerythritol | - |
Raphanus sativus | |
dithioerythritol | - |
Sinapis alba | |
dithiothreitol | - |
Raphanus sativus | |
dithiothreitol | - |
Sinapis alba | |
EDTA | - |
Raphanus sativus | |
EDTA | - |
Sinapis alba | |
Mn2+ | strong, above 5 mM | Raphanus sativus | |
Mn2+ | strong, above 5 mM | Sinapis alba | |
SDS | - |
Raphanus sativus | |
SDS | - |
Sinapis alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
1,2-Di-O-sinapoyl-beta-D-glucose | - |
Raphanus sativus | |
0.2 | - |
1,2-Di-O-sinapoyl-beta-D-glucose | - |
Sinapis alba | |
0.48 | - |
1-O-sinapoyl-beta-D-glucose | - |
Raphanus sativus | |
0.71 | - |
1-O-sinapoyl-beta-D-glucose | - |
Sinapis alba | |
5.3 | - |
choline | - |
Raphanus sativus | |
6.5 | - |
choline | - |
Sinapis alba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Raphanus sativus | |
additional information | no requirement for divalent cations | Sinapis alba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Raphanus sativus |
60000 | - |
gel filtration | Sinapis alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-sinapoyl-beta-D-glucose + choline | Raphanus sativus | involved in sinapine biosynthesis | D-glucose + sinapoylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + choline | Sinapis alba | involved in sinapine biosynthesis | D-glucose + sinapoylcholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
var. sativus cv. Saxa | - |
Sinapis alba | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Raphanus sativus |
partially | Sinapis alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | immature dark-green | Raphanus sativus | - |
seed | immature dark-green | Sinapis alba | - |
seed | 20% activity in mature seeds compared to immature seeds | Raphanus sativus | - |
seed | 20% activity in mature seeds compared to immature seeds | Sinapis alba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.258 | - |
partially purified enzyme | Sinapis alba |
0.486 | - |
partially purified enzyme | Raphanus sativus |
Storage Stability | Organism |
---|---|
-20°C, crude extract, 1 week, loss of 20% activity | Raphanus sativus |
-20°C, crude extract, 1 week, loss of 20% activity | Sinapis alba |
-20°C, partially purified, 2 weeks upon repeated freezing and thawing | Raphanus sativus |
-20°C, partially purified, 2 weeks upon repeated freezing and thawing | Sinapis alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-di-O-sinapoyl-beta-D-glucose + choline | best donor substrate | Raphanus sativus | 2-O-sinapoyl-beta-D-glucose + sinapoylcholine | - |
? | |
1,2-di-O-sinapoyl-beta-D-glucose + choline | best donor substrate | Sinapis alba | 2-O-sinapoyl-beta-D-glucose + sinapoylcholine | - |
? | |
1-feruloyl-beta-D-glucose + choline | - |
Raphanus sativus | D-glucose + feruloylcholine | - |
? | |
1-feruloyl-beta-D-glucose + choline | - |
Sinapis alba | D-glucose + feruloylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + choline | no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose | Raphanus sativus | D-glucose + sinapoylcholine | i.e. sinapine | ? | |
1-O-sinapoyl-beta-D-glucose + choline | no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose | Sinapis alba | D-glucose + sinapoylcholine | i.e. sinapine | ? | |
1-O-sinapoyl-beta-D-glucose + choline | absolute acceptor specificity towards choline | Raphanus sativus | D-glucose + sinapoylcholine | i.e. sinapine | ? | |
1-O-sinapoyl-beta-D-glucose + choline | absolute acceptor specificity towards choline | Sinapis alba | D-glucose + sinapoylcholine | i.e. sinapine | ? | |
1-O-sinapoyl-beta-D-glucose + choline | involved in sinapine biosynthesis | Raphanus sativus | D-glucose + sinapoylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + choline | involved in sinapine biosynthesis | Sinapis alba | D-glucose + sinapoylcholine | - |
? | |
1-p-coumaroyl-beta-D-glucose + choline | - |
Sinapis alba | D-glucose + p-coumaroylcholine | - |
? | |
1-p-coumaroyl-beta-D-glucose + choline | poor substrate | Raphanus sativus | D-glucose + p-coumaroylcholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCT | - |
Raphanus sativus |
SCT | - |
Sinapis alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Raphanus sativus |
45 | - |
- |
Sinapis alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Raphanus sativus |
7.6 | - |
- |
Sinapis alba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | - |
Raphanus sativus |
5.5 | 10 | - |
Sinapis alba |