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Literature summary for 2.3.1.91 extracted from

  • Gräwe, W.; Strack, D.
    Partial purification and some properties of 1-sinapoylglucose: choline sinapoyltransferase (sinapine synthase) from seeds of Raphanus sativus L. and Sinapis alba L. (1986), Z. Naturforsch. C, 41, 28-33.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong, above 5 mM Raphanus sativus
Ca2+ strong, above 5 mM Sinapis alba
Co2+ strong, above 5 mM Raphanus sativus
Co2+ strong, above 5 mM Sinapis alba
dithioerythritol
-
Raphanus sativus
dithioerythritol
-
Sinapis alba
dithiothreitol
-
Raphanus sativus
dithiothreitol
-
Sinapis alba
EDTA
-
Raphanus sativus
EDTA
-
Sinapis alba
Mn2+ strong, above 5 mM Raphanus sativus
Mn2+ strong, above 5 mM Sinapis alba
SDS
-
Raphanus sativus
SDS
-
Sinapis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
1,2-Di-O-sinapoyl-beta-D-glucose
-
Raphanus sativus
0.2
-
1,2-Di-O-sinapoyl-beta-D-glucose
-
Sinapis alba
0.48
-
1-O-sinapoyl-beta-D-glucose
-
Raphanus sativus
0.71
-
1-O-sinapoyl-beta-D-glucose
-
Sinapis alba
5.3
-
choline
-
Raphanus sativus
6.5
-
choline
-
Sinapis alba

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Raphanus sativus
additional information no requirement for divalent cations Sinapis alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Raphanus sativus
60000
-
gel filtration Sinapis alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-sinapoyl-beta-D-glucose + choline Raphanus sativus involved in sinapine biosynthesis D-glucose + sinapoylcholine
-
?
1-O-sinapoyl-beta-D-glucose + choline Sinapis alba involved in sinapine biosynthesis D-glucose + sinapoylcholine
-
?

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
var. sativus cv. Saxa
-
Sinapis alba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Raphanus sativus
partially Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
seed immature dark-green Raphanus sativus
-
seed immature dark-green Sinapis alba
-
seed 20% activity in mature seeds compared to immature seeds Raphanus sativus
-
seed 20% activity in mature seeds compared to immature seeds Sinapis alba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.258
-
partially purified enzyme Sinapis alba
0.486
-
partially purified enzyme Raphanus sativus

Storage Stability

Storage Stability Organism
-20°C, crude extract, 1 week, loss of 20% activity Raphanus sativus
-20°C, crude extract, 1 week, loss of 20% activity Sinapis alba
-20°C, partially purified, 2 weeks upon repeated freezing and thawing Raphanus sativus
-20°C, partially purified, 2 weeks upon repeated freezing and thawing Sinapis alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-di-O-sinapoyl-beta-D-glucose + choline best donor substrate Raphanus sativus 2-O-sinapoyl-beta-D-glucose + sinapoylcholine
-
?
1,2-di-O-sinapoyl-beta-D-glucose + choline best donor substrate Sinapis alba 2-O-sinapoyl-beta-D-glucose + sinapoylcholine
-
?
1-feruloyl-beta-D-glucose + choline
-
Raphanus sativus D-glucose + feruloylcholine
-
?
1-feruloyl-beta-D-glucose + choline
-
Sinapis alba D-glucose + feruloylcholine
-
?
1-O-sinapoyl-beta-D-glucose + choline no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
1-O-sinapoyl-beta-D-glucose + choline no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose Sinapis alba D-glucose + sinapoylcholine i.e. sinapine ?
1-O-sinapoyl-beta-D-glucose + choline absolute acceptor specificity towards choline Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
1-O-sinapoyl-beta-D-glucose + choline absolute acceptor specificity towards choline Sinapis alba D-glucose + sinapoylcholine i.e. sinapine ?
1-O-sinapoyl-beta-D-glucose + choline involved in sinapine biosynthesis Raphanus sativus D-glucose + sinapoylcholine
-
?
1-O-sinapoyl-beta-D-glucose + choline involved in sinapine biosynthesis Sinapis alba D-glucose + sinapoylcholine
-
?
1-p-coumaroyl-beta-D-glucose + choline
-
Sinapis alba D-glucose + p-coumaroylcholine
-
?
1-p-coumaroyl-beta-D-glucose + choline poor substrate Raphanus sativus D-glucose + p-coumaroylcholine
-
?

Synonyms

Synonyms Comment Organism
SCT
-
Raphanus sativus
SCT
-
Sinapis alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Raphanus sativus
45
-
-
Sinapis alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Raphanus sativus
7.6
-
-
Sinapis alba

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10
-
Raphanus sativus
5.5 10
-
Sinapis alba