Any feedback?
Literature summary for 2.3.1.9 extracted from
- Molecular cloning, biochemical characterization, and differential expression of an acetyl-CoA C-acetyltransferase gene (AACT) of Brahmi (Bacopa monniera) (2013), Plant Mol. Biol. Rep., 31, 547-557.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacopa monnieri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | inhibitory at concentrations higher than 5 mM | Bacopa monnieri |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02067 | - |
acetoacetyl-CoA | at pH 8.9 and 25°C | Bacopa monnieri | |
| 0.0258 | - |
acetyl-CoA | at pH 8.9 and 25°C | Bacopa monnieri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 5 mM | Bacopa monnieri | |
| additional information | not stimulated by Ni2+, Ca2+ and Zn2+ | Bacopa monnieri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41320 | - |
x * 41320, calculated from amino acid sequence | Bacopa monnieri |
| 43000 | - |
x * 43000, SDS-PAGE | Bacopa monnieri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 acetyl-CoA | Bacopa monnieri | - |
CoA + acetoacetyl-CoA | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacopa monnieri | D9U856 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 75 gel filtration | Bacopa monnieri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Bacopa monnieri | - |
| petal | highest expression in roots and petals | Bacopa monnieri | - |
| plant pedicel | - |
Bacopa monnieri | - |
| root | highest expression in roots and petals | Bacopa monnieri | - |
| sepal | - |
Bacopa monnieri | - |
| stem | - |
Bacopa monnieri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
crude enzyme, at pH 8.9 and 25°C | Bacopa monnieri |
| 56 | - |
after 62.22fold purification, at pH 8.9 and 25°C | Bacopa monnieri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 acetyl-CoA | - |
Bacopa monnieri | CoA + acetoacetyl-CoA | - |
r | |
| CoA + acetoacetyl-CoA | - |
Bacopa monnieri | 2 acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Bacopa monnieri |
| ? | x * 41320, calculated from amino acid sequence | Bacopa monnieri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AACT | - |
Bacopa monnieri |
| acetoacetyl-CoA C-acetyltransferase | - |
Bacopa monnieri |
| acetoacetyl-CoA thiolase | - |
Bacopa monnieri |
| thiolase II | - |
Bacopa monnieri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3833 | - |
acetoacetyl-CoA | at pH 8.9 and 25°C | Bacopa monnieri | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacopa monnieri | calculated from amino acid sequence | - |
6.2 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
