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Literature summary for 2.3.1.9 extracted from

  • Dyer, J.H.; Maina, A.; Gomez, I.D.; Cadet, M.; Oeljeklaus, S.; Schiedel, A.C.
    Cloning, expression and purification of an acetoacetyl CoA thiolase from sunflower cotyledon (2009), Int. J. Biol. Sci., 5, 736-744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
AACT, DNA and amino acid sequence determination and analysis, sequence comparison with Arabidopsis thaliana, expression of the active His-tagged enzyme in Escherichia coli TOP10 cells Helianthus annuus

Localization

Localization Comment Organism GeneOntology No. Textmining
glyoxysome
-
Helianthus annuus 9514
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, recombinant enzyme, SDS-PAGE Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus D2IH11
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged AACT 88fold to homogeneity by nickel affinity chromatography from Escherichia coli TOP10 cells Helianthus annuus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Helianthus annuus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.2
-
purified native enzyme Helianthus annuus
15.78
-
purified recombinant enzyme Helianthus annuus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + CoA coupled reaction assay in which the product of the thiolase reaction, acetyl-CoA, becomes the substrate for citrate synthase Helianthus annuus 2 acetyl-CoA
-
r

Subunits

Subunits Comment Organism
? x * 47000, recombinant enzyme, SDS-PAGE Helianthus annuus

Synonyms

Synonyms Comment Organism
AACT
-
Helianthus annuus
acetoacetyl CoA thiolase
-
Helianthus annuus
thiolase II
-
Helianthus annuus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Helianthus annuus