Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.9 extracted from

  • Kursula, P.; Ojala, J.; Lambeir, A.M.; Wierenga, R.K.
    The catalytic cycle of biosynthetic thiolase: a conformational journey of an acetyl group through four binding modes and two oxyanion holes (2002), Biochemistry, 41, 15543-15556.
    View publication on PubMed
Search for more literature for 2.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Zoogloea ramigera

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type thiolase and acetylated thiolase complexed with CoA, C89A mutant thiolase complexed with acetyl-CoA and acetoacetyl-CoA, Q64A mutant thiolase Zoogloea ramigera

Protein Variants

Protein Variants Comment Organism
C89A no thiolytic activity towards acetoacetyl-CoA Zoogloea ramigera
Q64A 30% lower kcat than that of the wild-type Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Zoogloea ramigera
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera P07097
-
-

Reaction

Reaction Comment Organism Reaction ID
2 acetyl-CoA = CoA + acetoacetyl-CoA reaction involves 2 chemically distinct steps: acetyl group transfer from acetyl-CoA to Cys89, and transfer of this acetyl group to a second acetyl-CoA in the Claisen condensation step to form acetoacetyl-CoA Zoogloea ramigera
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 r