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Literature summary for 2.3.1.9 extracted from

  • Nishimura, T.; Saito, T.; Tomita, K.
    Purification and properties of beta-ketothiolase from Zoogloea ramigera (1978), Arch. Microbiol., 116, 21-27.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 0.4 mM, 87% inhibition Zoogloea ramigera
iodoacetamide 0.4 mM, 36% inhibition Zoogloea ramigera
N-ethylmaleimide 0.4 mM, 53% inhibition Zoogloea ramigera
NADH inhibition of thiolysis Zoogloea ramigera
NADPH
-
Zoogloea ramigera
p-chloromercuribenzoate 0.4 mM, 95% inhibition Zoogloea ramigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
CoA
-
Zoogloea ramigera
0.01
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.33
-
acetyl-CoA
-
Zoogloea ramigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000, SDS-PAGE Zoogloea ramigera
190000
-
gel filtration Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Zoogloea ramigera
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200 Zoogloea ramigera

Reaction

Reaction Comment Organism Reaction ID
2 acetyl-CoA = CoA + acetoacetyl-CoA bi bi ping pong mechanism Zoogloea ramigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91
-
-
Zoogloea ramigera

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA
-
Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Zoogloea ramigera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
condensation Zoogloea ramigera
8.5
-
thiolysis Zoogloea ramigera

pH Range

pH Minimum pH Maximum Comment Organism
6 9 approx. 80% of maximal activity at pH 6.0, approx. 40% at pH 9.0 Zoogloea ramigera
6.5 9.2 approx. 50% of maximal activity at pH 6.5 and pH 9.2, thiolysis Zoogloea ramigera