Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 0.4 mM, 87% inhibition | Zoogloea ramigera | |
iodoacetamide | 0.4 mM, 36% inhibition | Zoogloea ramigera | |
N-ethylmaleimide | 0.4 mM, 53% inhibition | Zoogloea ramigera | |
NADH | inhibition of thiolysis | Zoogloea ramigera | |
NADPH | - |
Zoogloea ramigera | |
p-chloromercuribenzoate | 0.4 mM, 95% inhibition | Zoogloea ramigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
CoA | - |
Zoogloea ramigera | |
0.01 | - |
acetoacetyl-CoA | - |
Zoogloea ramigera | |
0.33 | - |
acetyl-CoA | - |
Zoogloea ramigera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Zoogloea ramigera |
190000 | - |
gel filtration | Zoogloea ramigera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Zoogloea ramigera | - |
acetyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200 | Zoogloea ramigera |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 acetyl-CoA = CoA + acetoacetyl-CoA | bi bi ping pong mechanism | Zoogloea ramigera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
91 | - |
- |
Zoogloea ramigera |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | - |
Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE | Zoogloea ramigera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
condensation | Zoogloea ramigera |
8.5 | - |
thiolysis | Zoogloea ramigera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | approx. 80% of maximal activity at pH 6.0, approx. 40% at pH 9.0 | Zoogloea ramigera |
6.5 | 9.2 | approx. 50% of maximal activity at pH 6.5 and pH 9.2, thiolysis | Zoogloea ramigera |