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Literature summary for 2.3.1.9 extracted from
- A Streptomyces collinus thiolase with novel acetyl-CoA:acyl carrier protein transacylase activity (2001), Biochemistry, 40, 11955-11964.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces collinus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C92S | kcat decreases to 0.2% of that for the recombinant thiolase | Streptomyces collinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiolactomycin | 0.36 mM, 50% inhibition | Streptomyces collinus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
acetoacetyl-CoA | native thiolase | Streptomyces collinus | |
| 0.0062 | - |
acetoacetyl-CoA | C92S mutant thiolase | Streptomyces collinus | |
| 0.0098 | - |
acetoacetyl-CoA | recombinant thiolase | Streptomyces collinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
native thiolase, gel filtration | Streptomyces collinus |
| 187000 | - |
recombinant thiolase, gel filtration | Streptomyces collinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Streptomyces collinus | - |
acetyl-CoA + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces collinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, ammonium sulfate, Sephacryl S-200, Mono Q, FPLC S-300 | Streptomyces collinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.6 | - |
- |
Streptomyces collinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Streptomyces collinus | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | enzyme has both thiolase and acetyl-CoA:acyl carrier protein transacylase activity | Streptomyces collinus | acetyl-CoA + acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 46000-48000, recombinant thiolase, SDS-PAGE | Streptomyces collinus |
| tetramer | 4 * 45000-47000, native thiolase, SDS-PAGE | Streptomyces collinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
15 min, loss of 47% activity | Streptomyces collinus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0767 | - |
acetoacetyl-CoA | C92S mutant thiolase | Streptomyces collinus | |
| 36.2 | - |
acetoacetyl-CoA | native thiolase | Streptomyces collinus | |
| 40.1 | - |
acetoacetyl-CoA | recombinant thiolase | Streptomyces collinus | |
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Release 2023.1 (January 2023)
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